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Royal Society of Chemistry-12 Aug 2019 圣草次苷

時(shí)間：2020-09-03   瀏覽次數(shù)：  分享到：

A comprehensive study of eriocitrin metabolism in vivo and in vitro based on an efficient UHPLC-Q-TOF-MS/MS strategy

期刊名：Royal Society of Chemistry
文獻(xiàn)編號(hào)：DOI 10.1039/C9RA03037A
文獻(xiàn)地址：https://pubs.rsc.org/en/content/articlelanding/2019/RA/C9RA03037A#!divAbstract
發(fā)表日期：12 Aug 2019

Abstract
Eriocitrin, a main flavonoid in lemons, possesses strong antioxidant, lipid-lowering and anticancer activities and has long been used in food, beverages and wine. However, its metabolism in vivo and in vitro is still unclear. In this study, an efficient strategy was developed to detect and identify metabolites of eriocitrin by using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) based on online data acquisition and multiple data processing techniques. A total of 32 metabolites in vivo and 27 metabolites in vitro were obtained based on the above method. Furthermore, the main metabolic pathways of eriocitrin included reduction, hydrogenation, N-acetylation, ketone formation, oxidation, methylation, sulfate conjugation, glutamine conjugation, glycine conjugation, desaturation and demethylation to carboxylic acid. This study will lay a foundation for further studies on the metabolic mechanisms of eriocitrin.

Eriocitrin (13463-28-0, purity > 98.5%) was purchased  from Chengdu Desite Biological Technology Co. Ltd (Chengdu, China)

 

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