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One-Way Intestinal Perfusion of PVP/VA–Poloxamer ...

時(shí)間：2022-05-30 瀏覽次數(shù)：分享到：

Turmeric was the dried rhizome of Curcuma longa L., and its extract had important pharmacological effects such as anti-tumor, cholagogic, and antioxidant. However,curcuma extract had poor water solubility and low bioavailability, which had become the main limiting factor for its clinical application. The purpose of this study was to prepare PVP/VA–Poloxamer–188–curcuma extract solid dispersion (PAP–CSD) to improve the solubilityand bioavailability of the curcuma extract. The intestinal absorption mechanism of solid dispersion of this extract was studied by one-way intestinal perfusion in rats. PAP–CSD,PVP/VA–curcuma extract solid dispersion (PA–CSD) and Poloxamer–188–curcuma extract solid dispersion (P–CSD) was able to improve the intestinal absorption of the curcuma extract (P< 0.05), and PAP–CSD (combined use of two carriers) was better than that of PA–CSD and P–CSD. CCK8 method was used to investigate the effects of the curcuma extract and PAP–CSD on the proliferation of hepatic stellate cells (HSC)–T6 cells. The inhibitory effect ofPAP–CSD on the proliferation of HSC-T6 cells, related to the p38 MAPK pathway, was better than that of the curcuma extract.

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Materials

Bisdemethoxycurcumin reference substance, demethoxycurcumin reference substance, and curcumin reference substance were all purchased from Chengdu DST Biotechnology Co., Ltd. (batch number: DST191009-021, DST190910-030, DST200412-014),

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